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Literature summary extracted from

  • Rajakovich, L.; Pandelia, M.; Mitchell, A.; Chang, W.; Zhang, B.; Boal, A.; Krebs, C.; Bollinger, J.
    A new microbial pathway for organophosphonate degradation catalyzed by two previously misannotated non-heme-iron oxygenases (2019), Biochemistry, 58, 1627-1647 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.90 expressed in Escherichia coli BL21(DE3) cells Leisingera caerulea
1.14.11.72 expressed in Escherichia coli BL21(DE3) cells Leisingera caerulea

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.90 in complex with Fe2+ and [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate, hanging drop vapor diffusion method, using 0.2 M CaCl2, 0.1 M sodium HEPES (pH 7.5), and 27-33% polyethylene glycol 4000 Leisingera caerulea
1.14.11.72 in complex with Fe2+, [2-(trimethylamino)ethyl]phosphonate and 2-oxoglutarate, hanging drop vapor diffusion method, using 1.0-1.4 M Li2SO4 and 1.6-1.8 M (NH4)2SO4 Leisingera caerulea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.78 Fe2+ dependent on uncultured bacterium HF130_AEPn_1
1.13.11.90 Iron the enzyme uses a mixed-valent diiron cofactor Leisingera caerulea
1.14.11.72 Fe2+ dependent on Leisingera caerulea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.72 70000
-
 gel filtration Leisingera caerulea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.78 (2-amino-1-hydroxyethyl)phosphonate + O2 uncultured bacterium HF130_AEPn_1
-
 glycine + phosphate
-
 ?
1.13.11.90 [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + O2 Leisingera caerulea
-
 glycine betaine + phosphate
-
 ?
1.14.11.72 [2-(trimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2 Leisingera caerulea
-
 [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.78 uncultured bacterium HF130_AEPn_1 D0E8I5
-
-
1.13.11.90 Leisingera caerulea A0A4V8H040
-
-
1.14.11.72 Leisingera caerulea A0A4V8H042
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.90 Ni-NTA column chromatography and Sephadex S-200 gel filtration Leisingera caerulea
1.14.11.72 Ni-NTA column chromatography and Sephadex S-200 gel filtration Leisingera caerulea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.78 (2-amino-1-hydroxyethyl)phosphonate + O2
-
 uncultured bacterium HF130_AEPn_1 glycine + phosphate
-
 ?
1.13.11.90 [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + O2
-
 Leisingera caerulea glycine betaine + phosphate
-
 ?
1.14.11.72 2-aminoethylphosphonate + 2-oxoglutarate + O2
-
 Leisingera caerulea ?
-
 ?
1.14.11.72 3-aminopropylphosphonate + 2-oxoglutarate + O2
-
 Leisingera caerulea ?
-
 ?
1.14.11.72 additional information no activity with gamma-butyrobetaine or analogue substrates Leisingera caerulea ?
-
-
1.14.11.72 phosphocholine + 2-oxoglutarate + O2
-
 Leisingera caerulea glycine betaine aldehyde + phosphate + succinate + CO2
-
 ?
1.14.11.72 [2-(dimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2
-
 Leisingera caerulea [(1R)-1-hydroxy-2-(dimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2
-
 ?
1.14.11.72 [2-(trimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2
-
 Leisingera caerulea [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.72 homodimer 2 * 41000, calculated from amino acid sequence Leisingera caerulea

Synonyms

EC Number Synonyms Comment Organism
1.13.11.78 (R)-1-hydroxy-2-aminoethylphosphonate oxygenase
-
 uncultured bacterium HF130_AEPn_1
1.13.11.78 phnZ
-
 uncultured bacterium HF130_AEPn_1
1.13.11.90 tmpB
-
 Leisingera caerulea
1.14.11.72 gamma-butyrobetaine hydroxylase misannotation Leisingera caerulea
1.14.11.72 tmpA
-
 Leisingera caerulea

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.72 2-oxoglutarate dependent on Leisingera caerulea